That sounds strange... I've attached some cool steps for great guitar tuning. This will help you out for sure!
Tips
* After tuning all strings from the 6th to the 1st, check the 6th string pitch again. Most probably it will go out of tune because you changed strings tension and the neck changed its shape, making all strings shorter or longer. This is especially the case for acoustic guitars. If this happened, tune your guitar again.
* Harmonic tuning is more accurate and easier for some people. This is done by comparing the harmonic on the fifth fret of the lower string to that of the seventh fret on the higher string. To play a harmonic, lightly touch the string directly above the fret and let it go as you pluck the string. Another advantage of harmonic tuning is that both strings will continue to ring as you let go of the neck to adjust the tuning pegs. Note: this doesn't work for the second (B) string.
* Tune your guitar every time you use it. Playing can make your guitar go out of tune, especially if you have a cheap guitar or old, cheap strings or if you use tremolo a lot.
* If you are tuning a bass guitar, the layout is the same. The difference is a bass guitar doesn't have a B and high E string.
* You will find it easier to tune each string if you loosen the string to flatten the note, then increase the tension to come up to pitch. Some of the strings have a tendency to "stick" where they slide over the nut, and will be in tune until they suddenly slip flat. By coming up to pitch, this tendency is avoided. You can also lubricate the slot in the nut with graphite (pencil lead) and it will help to avoid sticking during tuning.
* If you don't have access to a piano, keyboard, or tuner, pick up your phone! In the US at least, standard dial tone is an F. Also, You can use a tuning fork To tune a guitar, in a pinch.
* After tuning, strum open major chords in the five common chord forms (C, F, G, A, and D). Make sure they sound right (no dissonance, or wavering). A guitar can seem in tune using the method described above, but if the intonation is not set up quite right the guitar won't sound quite right. You may need to make some minor adjustments to ensure that chords sound right.
* After tuning, make sure the G string and the G at the eighth fret of the B string sound the same (other than being an octave apart, obviously). These two notes being slightly out of tune is a common problem and will make chords sound very bad. It's better to have the E and B strings a *little* bit flat relative to the low E string than to have the G string sound out of tune with the E and B strings. This problem has to do with the way guitars are designed but is exacerbated by bad intonation.
* Tune your guitar to concert pitch. Tuning it flat can have a negative subconscious effect on your listeners. Most people have a sense of absolute pitch whether they recognize it or not. Tuning your guitar flat will make something sound not quite right to your listeners. Besides, playing trains your ears. Train them right!
* If you have a Floyd Rose or other floating bridge, and you have a locking nut, then the little twisty knobs at the bottom tune your guitar. Clockwise is up, and counter-clockwise is down. Then again, if you have a Floyd Rose you are probably proficient enough to not be looking at this page.
* Also, if you have a Floyd Rose trem, note that switching between tunings requires you to adjust the springs in the back of the guitar to achieve proper string tension and tremolo placement. If you tune down to drop-c and you neglect this adjustment, your trem will bottom out and may even pull your strings down over the pickups, making you extremely frustrated. If you come back up to standard from drop-c tuning, your trem will be pulled waaaaay off of the body. I learned all this the hard way. It scared the heck out of me!
* If you have trouble remembering the notes for the strings, think, "Every Afternoon Dad Goes, Buys Eggplants." (Going from low E to high E) or 'Every Body Gets Dinner At Eight' (Going from high E to low E).
* If you have recently changed your guitar strings, the guitar will go out of tune faster and you will have to retune more often. As you play, and the strings break in, you will find you have to tune the guitar less often, but there's a way to fix this right after you put them on. When you tune a string right after you put it on, "stretch" the string. By stretch I mean pull the string gently towards the bridge. You will notice that it goes out of tune quickly. Just tune it up again and stretch and when it doesn't go out of tune anymore. That means it's perfect.